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1987年,由Selker等在粗糙脉孢菌中首次发现重复序列诱导点突变(repeat-induced point mutation,RIP).在重复序列诱导点突变过程中,搜寻前减数分裂组织单倍体核中DNA的重复序列,然后发生众多的碱基C到T的突变,产生富碱基T+A片段,从而使重复序列中的G-C碱基对发生转换突变成为A-T碱基对.此外,发生RIP的序列多集中在着丝粒区域,主要是转座子甲基化后的遗迹.移动转座子是真核生物基因组进化的主要驱动力.对于真菌,重复序列诱导点突变(RIP)在减数分裂过程中通过突变多拷贝DNA,能最大限度地减少转座子的影响,因此对RIP的研究在一定程度上能有助于了解基因组进化的真谛.综述了重复序列诱导点突变的产生机制,以及真菌中重复序列诱导点突变的研究进展.
Abstract:Repeat- induced point mutations( RIP) was discovered in Neurospora crassa in 1987 by Selker.RIP searches for sequence duplications in haploid nuclei of premeiotic tissue and then litters them with numerous C to T mutations. T + A rich fragments so that the G- C pairs in duplications can be mutated to A- T. In addition,RIP's sequences,which are concentrated in centromeric regions,and are predominantly relics of transposons,are left methylated. Mobile transposable elements are among the primary drivers of the evolution of eukaryotic genomes.For fungi,repeat- induced point mutation( RIP) silencing minimizes deleterious effects of transposons by mutating multicopy DNA during meiosis. To explore the impact of RIP- mutated transposons is conducive to generate evolutionary inferences for phylogenetic and population genetic analyses. The paper has reviewed the mechanism of RIP and the progress of RIP in fungi.
[1]Selker E U,Cambareri E B,Jensen B C,et al.Rearrangement of duplicated DNA in specialized cells of Neurospora[J].Cell,1987,51(5):741-752.
[2]Galagan J E,Selker E U.RIP:the evolutionary cost of genome defense[J].Trends Genet,2004,20(9):417-423.
[3]Selker E U.Premeiotic instability of repeated sequences in Neurospora crassa[J].Rev Genet,1990,24(1):579-613.
[4]Watters M K,Randall T A,Margolin B S,et al.Action of repeat-induced point mutation on both strands of a duplex and on tandem duplications of various sizes in Neurospora[J].Genetics,1999,153(2):705–714.
[5]Cambareri E B,Singer M J,Selker E U.Recurrence of repeat-induced point mutation(RIP)in Neurospora crassa[J].Genetics,1991,127(4):699–710.
[6]Cambareri E B,Jensen B C,Schabtach E,et al.Repeatinduced G-C to A-T mutations in Neurospora[J].Science,1989,244(4912):1571–1575.
[7]Irelan J T,Hagemann A T,Selker E U.High frequency repeat-induced point mutation(RIP)is not associated with efficient recombination in Neurospora[J].Genetics,1994,138(4):1093–1103.
[8]Borkovich K A,Alex L A,Yarden O,et al.Lessons from the genome sequence of Neurospora crassa:tracing the path from genomic blueprint to multicellular organism[J].Mol.Biol.Rev,2004,68(1):1–108.
[9]Crouch J A,Glasheen B M,Giunta M A,et al.The evolution of transposon repeat-induced point mutation in the genome of Colletotrichum cereale:reconciling sex,recombination and homoplasy in an“asexual”pathogen[J].Fungal Genet Biol.,2008,45(3):190-206.
[10]Ikeda K,Nakayashika H,Kataoka T,et al.Repeat-in-duced point mutation(RIP)in Magnaporthe grisea:implications for its sexual cycle in the natural field context[J].Mol.Biol.,2002,45(5):1355–1364.
[11]Freitag M,Williams R L,Kothe G O,et al.A cytosine methyltransferase homologue is essential for repeat-induced point mutation in Neurospora crassa[J].Proc.Natl.Acad.Sci.USA.,2002,99(13):8802–8807.
[12]Mautino M R,Rosa A L.Analysis of models involving enzymatic activities for the occurrence of C/T transition mutations during repeat-induced point mutation(RIP)in Neurospora crassa[J].J.The.Biol.,1998,192(1):61–71.
[13]Machida M,Asai K,Sano M,et al.Genome sequencing and analysis of Aspergillus oryzae[J].Nature,2005,438(4):1157–1161.
[14]Foss H M,Selker E U.Efficient DNA pairing in a Neurospora mutant defective in chromosome pairing[J].Mol.Gen.Genet.,1991,231(1):49–52.
[15]Bowring F J,Catchesaid D E.The effect of rec-2 on repeat induced point-mutation(RIP)and recombination events that excise DNA sequence duplications at the his-3locus in Neurospora crassa[J].Genetics,1993,23(5-6):496–500.
[16]Kouzminova E A,Selker E U.dim-2 encodes a DNA methyltransferase responsible for all known cytosine methylation in Neurospora[J].EMBO J,2001,20(15):4309-4323.
[17]Watters M K,Randall T A,Margolin B S,et al.Action of repeat induced point mutation on both strands of a duplex and on tandem duplications of various sizes in Neurospora[J].Genetics,1999,153(2):705-714.
[18]Tamaru H,Selker E U.Synthesis of signals for de novo DNA methylation in Neurospora crassa[J].Mol.Cell.Biol,2003,23(7):2379-2394.
[19]Singer M J,Marcotte B A,Selker E U.DNA methylation associated with repeat induced point mutation in Neurospora crassa[J].Mol.Cell,Biol.,1995,15(10):5586–5597.
[20]Aramayo R,Selker E U.Neurospora crassa,a Model System for Epigenetics Research[J].Cold Spring Harbor Biology,2013,5:a017921.
[21]Selker E U,Stevens J N.DNA methylation at asymmetric sites is associated with numerous transition mutations[J].Proc.Natl.Acad.Sci.USA.,1985,82(23):8114–8118.
[22]邱立友,余翠,戚元成,等.真菌表观遗传学研究进展[J].Chinese Journal of Cell Biology,2009,31(2):212-216.
[23]Selker E U.Pinstability of repeated sequences in Neurospora crassa[J].Rev Genet,1990,24(1):579-613.
[24]Cambareri E B,Aisner R,Carbon J.Structure of the chromosome VII centromere region in Neurospora crassa:degenerate transposons and simple repeats[J].Mol.Cell.Biol.,1998,18(9):5465–5477.
[25]Selker E U,Cross S H,Murphy J G,et al.The methylated component of the Neurospora crassa genome[J].Nature,2003,422:893–897.
[26]Borkovich K A,Alex L A,Yarden O,et al.Lessons from the genome sequence of Neurospora crassa:tracing the path from genomic blueprint to multicellular organism[J].Mol.Biol.Rev.,2004,68(1):1–108.
[27]Galagan J E,Calvo S E,Borkovich K A,et al.The genome sequence of the filamentous fungus Neurospora crassa[J].Nature,2003,422:859–868.
[28]Selker E U.Epigenetic phenomena in filamentous fungi:useful paradigms or repeat-induced confusion[J].Trends Genet,1997,13(8):296-301.
[29]Clutterbuck A J.Genomic evidence of repeat-induced point mutation(RIP)in filamentous ascomycetes[J].Fungal Genetics and Biology,2011,48(3):306–326.
[30]Ikeda K I,Nakayashika H,Kataoka T,et al.Repeat-induced point mutation(RIP)in Magnaporthe grisea:implications for its sexual cycle in the natural field context[J].Mol.Biol.,2002,45(3):1355–1364.
[31]Shiflett A M,Enkerli J,Covert S F,et al.Nht2,a copia LTR retrotransposon from a conditionally dispensible chromosome in Nectria haematococca[J].Genetics,2002,41(2):99–106.
[32]Graia F,Lespinet O,Rimbault B,et al.Genome quality control:RIP(repeat-induced point mutation)comes to Podospora[J].Mol.Biol.,2001,40(3):586–595.
[33]Hamann A,Feller F,Osiewacz H D.The degenerate DNA transposon Pat and repeat-induced point mutation(RIP)in Podospora anserine[J].Mol.Gen.Genet.,2000,263(6):1061–1069.
[34]Arnaise S,Zickler D,Bourdais A,et al.Mutations in mating-type genes greatly decrease repeat-induced point mutation process in the fungus Podospora anserine[J].Fungal Genet.Biol.,2008,45(2):207–220.
[35]Hua-van A,Langin T,Daboussi M J.Evolutionary history of the impala transposon in Fusarium oxysporum[J].Mol.Biol.Evol.,2001,18(10):1959–1969.
[36]Daboussi M J,Daviere J M,Grazione S,et al.Evolution of the Fot1 transposons in the genus Fusarium:dicontinuous distribution and epigenetic inactivation[J].Mol.Biol.Evol.,2002,19(4):510–520.
[37]Coleman J,Rounsley S D,Wasmann C,et al.The Genome of Nectria haematococca:contribution of supernumerary chromosomes to gene expansion[J].PloS Genet.,2009,5:1000618.
[38]Ma L J,Borkovich K A,Coleman J,et al.Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium[J].Nature,2010,464:367–373.
[39]Clutterbuck A J.MATE transposable elements in Aspergillus nidulans:evidence of repeat-induced point mutation[J].Fungal Genet,Biol.,2004,41(3):308–316.
[40]Montiel M D,Lee H A,Archer D B.Evidence of RIP(repeat-induced point mutation)in transposase sequences of Aspergillus oryzae[J].Fungal Genet,Biol.,2006,43(6):439–445.
[41]Clutterbuck A J,Kapitonov V,Jurka J,et al.Transposable elements and repeat induced point mutation in Aspergillus nidulans,Aspergillus fumigatus and Aspergillus oryzae[J].Fungal Genet,Biol.,2008,36(2):343–355.
[42]Hey P,Robson G,Birch M,et al.Characterization of Aft1 a Fot/Pogo type transposon of Aspergillus fumigatus[J].Fungal Genet,Biol.,2008,45(6):117–126.
[43]Machida M,Asai K,Sano M,et al.Genome sequencing and analysis of Aspergillus oryzae[J].Nature,2005,438:1157–1161.
[44]Ogasawara H,Obata H,Hata Y,et al.Crawler,a novel Tc1/Mariner-type transposable element in Aspergillus oryzae transposes under stress conditions[J].Fungal Genet,Biol.,2009,46(6-7):441–449.
[45]Braumann I,Van-den B M,Kempken F.Repeat induced point mutation in two asexual fungi,Aspergillus niger and Penicillium chrysogenum[J].Cur.Genet.,2008,53(5):287–297.
[46]Idnurm A,Howlett B J.Analysis of loss of pathogenicity mutants reveals that repeat-induced point mutations can occur in the Dothideomycete Leptosphaeria maculans[J].Fungal Genet,Biol.,2003,39(1):31–37.
[47]Attard A,Gout L,Ross S,et al.Truncated and RIP-degenerated copies of the LTR retrotransposon Pholy are clustered in a pericentromeric region of the Leptosphaeria maculans genome[J].Fungal Genet,Biol.,2005,42(1):30–41.
[48]Isabelle F,Simon R,Hortense B,et al.Repeat-Induced Point Mutation(RIP)as an Alternative Mechanism of Evolution Toward Virulence in Leptosphaeria maculans[J].The American Phytopathological Society,2009,22(8):932-941.
[49]Rouxel T,Grandaubert J,Hane J K,et al.Effector diversification within compartments of the Leptosphaeria maculans genome affected by Repeat-Induced Point mutations[J].Nature,2011,15(10):1–10.
[50]Horns F,Petit E,Hood M E,et al.Patterns of RepeatInduced Point Mutation in Transposable Elements of Basidiomycete Fungi[J].Genome Biol.Evol.,2012,4(3):240–247.
基本信息:
DOI:10.16393/j.cnki.37-1436/z.2014.05.009
中图分类号:Q933
引用信息:
[1]冯凤鹃,曲志才,田李,等.真菌中DNA重复序列诱导点突变的研究进展[J].菏泽学院学报,2014,36(05):54-61.DOI:10.16393/j.cnki.37-1436/z.2014.05.009.
基金信息:
中国博士后科学基金资助项目(2013M531567);; 山东省自然科学基金资助项目(ZR2013CQ018)