李稳,杜晶晶,牟惠冰,王晓敏,周广灿

• 1:菏泽学院农业与生物工程学院(牡丹学院)
• 2:菏泽学院牡丹研究院

摘要(Abstract)：

牡丹具有产油率高，富含亚麻酸、亚油酸和油酸等超长链不饱和脂肪酸含量高等特点，是一种优质的木本油料作物.然而，牡丹基因组中KCS基因的存在情况及其演化特征鲜有报道.从牡丹基因组中鉴定出13条KCS基因，蛋白质的氨基酸序列长度在274～967aa之间，它们编码的蛋白质中均含有3-Oxoacyl-[acyl-carrier-protein(ACP)]synthaseⅢC结构域和TypeⅢpolyketide synthase-like结构域.系统发育分析显示，牡丹KCS基因组成4个演化分支.同一演化分支或近缘演化分支中的KCS基因编码蛋白质的结构域和氨基酸基序相似，而演化关系较远演化分支中的基因结构差异较大.研究牡丹KCS基因的演化特征能进一步发掘牡丹KCS基因的功能，加深对其超长链脂肪酸合成机制的了解，为油用牡丹的分子遗传育种提供一定的理论指导.

关键词(KeyWords)： 牡丹基因组;β-酮酯酰-CoA合酶(KCS)基因;鉴定;演化分析

Abstract:

Keywords:

基金项目(Foundation): 菏泽学院科研基金项目(XY19BS15)

作者(Author): 李稳,杜晶晶,牟惠冰,王晓敏,周广灿

DOI: 10.16393/j.cnki.37-1436/z.2022.02.028

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