姜曰水,曲志才

• 1:曲阜师范大学生命科学学院

摘要(Abstract)：

Wolbachia是当前分布最广的共生菌类,参与调控寄主的多种生殖活动.通过对山东8个地理种群野生果蝇Wolbachia的WSP基因进行检测,在5个地理种群的黑腹果蝇和3个地理种群拟果蝇中分别分离到长度为632bp和611bp的Wolbachia的WSP基因片段,命名为WSPSD632和WSPSD611,在GeneBank所获注册号分别为EU395833和EU395834.将两序列和GeneBank上公布的WSP基因序列构建系统发育树,表明山东野生黑腹果蝇和拟果蝇所感染的Wolbachia分属于WMel和WRi类群.序列比对结果显示,两核苷酸序列的同源性为86.39%,但碱基替换率和缺失率存在一定差异.

关键词(KeyWords)： 山东;野生果蝇;Wolbachia;WSP基因;序列分析

Abstract:

Keywords:

基金项目(Foundation): 曲阜师范大学科研基金资助项目(XJ0613)

作者(Author): 姜曰水,曲志才

DOI: 10.16393/j.cnki.37-1436/z.2008.05.013

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