薄层色谱-紫外可见分光光度法测定食品中的苏丹红Ⅲ、ⅣSimultaneous Determination of Sudan Red Ⅲand Sudan Red Ⅳ in Foods by TLC-UV-Vis Spectrophotometer
庞艳玲
摘要(Abstract)：
建立了测定食品中苏丹红Ⅲ、Ⅳ含量的新方法——薄层色谱-紫外可见分光光度法,讨论了影响测定的各种因素,方法的线性回归方程是苏丹红Ⅲ:A=0.09096c-0.00354,R=0.999;苏丹红Ⅳ:A=0.09298c-0.01514,R=0.999;线性范围:苏丹红Ⅲ:0.5~15μg/mL,苏丹红Ⅳ:0.5~15μg/mL;检出限:苏丹红Ⅲ、Ⅳ均为:0.05μg/mL.经过对实际样品的检测和与其它检测方法的比较,证明该方法具有良好的重现性,简便、准确,适合苏丹红的日常检测.
关键词(KeyWords)： 苏丹红;薄层分离;紫外可见分光光度法;超声萃取
基金项目(Foundation): 菏泽学院科学研究基金资助项目(XY07HX01)
作者(Author): 庞艳玲
DOI: 10.16393/j.cnki.37-1436/z.2008.02.013
参考文献(References)：
- [1]卫生部.苏丹红危险性评估报告[J].中国食品卫生杂志,2005,17(5):276-278.
- [2]Stiborov偄M,Martinek V,Rydlov偄H,et al.Hodek,Ex-pression of cytochrome P450 1A1 and its contribution tooxidation of a potential human carcinogen 1-phenylazo-2-naphthol(SudanⅠ)in human livers[J].Cancer Let-ters,2005,220(2):145-155.
- [3]GB/T19681-20051,食品中苏丹红染料的检测方法高效液相色谱法[S].
- [4]谢维平,黄盈煜,傅晖蓉,等.凝胶柱净化-高效液相色谱检测食品中的苏丹红[J].色谱,2005,23(5):542.
- [5]Calbiani F,CareriM,Elviri L,et al.Accurate mass meas-urements for the confirmation of Sudan azo-dyes in hotchilli products by capillary liquid chromatography-elec-trospray tandem quadrupole orthogonal-acceleration timeof flight mass spectrometry[J].J Chromatogr A,2004,1058(12):127.
- [6]Tateo F,Bononi M.Fast Determination of SudanⅠbyHPLC/APCI-MS in Hot Chilli,Spices,and Oven-Baked Foods[J].J Agric Food Chem,2004,52(4):655-6581.
- [7]Donna L D,Maiuolo L,Mazzotti F.Assay of SudanⅠcontamination of foodstuff by atmospheric pressure chemi-cal ionization tandem mass spectrometry and isotope dilu-tion[J].Anal Chem,2004,76(17):5104.
- [8]郑用熙.分析化学中的数理统计方法[M].北京:科学出版社,1986:122,231,308.