条斑紫菜叶状体嵌合性的分子生物学证据Evidence in Molecular Biology on Mosaic Thallus in Porphyra Yezoensis
王娟
摘要(Abstract)：
利用微卫星标记的共显性特点,选用5对微卫星引物对65个条斑紫菜叶状体个体的嵌合性,通过聚丙烯酰胺凝胶电泳进行了分析。结果显示:聚丙烯酰胺凝胶电泳中既有两条带又有一条带,也即条斑紫菜叶状体个体中既有嵌合体又有纯合体,其中58.5%为纯合体,41.5%为嵌合体。这为条斑紫菜叶状体的嵌合性提供了分子生物学方面的证据。
关键词(KeyWords)： 条斑紫菜;嵌合体;微卫星;聚丙烯酰胺凝胶电泳;DNA
基金项目(Foundation):
作者(Author): 王娟
DOI: 10.16393/j.cnki.37-1436/z.2008.05.014
参考文献(References)：
- [1]Miura A,Kunifuji Y.Genetic analysis of the pigmenta-tion types in the seaweed Susabi-nori(Porphyra yezoen-sis)[J].Iden(Heredity),1980,34:14-20.
- [2]Ohme M,Kunifuji Y.Cross experiments of the color mu-tants in Porphyra yezoensis Ueda[J].Jap.J.Phycol.,1986,34:101-106.
- [3]Ohme M,Miura A.Tetrad analysis in conchospore germ-lings of Porphyra yezoensis(Rhodophyta,Bangiales)[J].Plant Science,1988,57:135-140.
- [4]Niwa K,Miura A,Shin J A,et al.Characterization andgenetic analysis of the violet type pigmentation mutant ofPorphyra yezoensis(Bangiaceae,Rhodophyta)[J].Ko-rean J.Phycol.,1993,8:217-230.
- [5]Niwa K,Mizuta A,Aruga Y.Genetic characterization ofa spontaneous green-type pigmentation mutant of Por-phyra yezoensis and the signigicance of using heterozygousconchocelis in nori farming[J].Fisheries Science,2002,68:729-735.
- [6]严兴洪,田中次郎,有贺佑胜.条斑紫菜色彩突变体的诱导、分离和特性分析[J].水产学报,2000,24(3):221-228.
- [7]Yan X,Aruga Y.Genetic analysis of artificial pigmenta-tion mutants in Porphyra yezoensis Ueda(Bangiales,Rhodophyta)[J].Phycological Research 2000,48:177-187.
- [8]严兴洪,梁志强,宋武林,等.坛紫菜人工色素突变体的诱变与分离[J].水产学报,2005,29(2):165-172.
- [9]许璞,费修绠,张学成,等.紫菜色素突变体诱导的研究-I.NG对紫菜壳孢子诱变的效果及遗传分析[J].海洋通报,2002,21(5):19-25.
- [10]许璞,费修绠,张学成,等.紫菜色素突变体诱导的研究-II.NG对紫菜叶状体诱变的效果及遗传分析[J].海洋通报,2003,22(1):24-29.
- [11]刘必谦,曾庆国,骆其君,等.条斑紫菜(Porphyrayezoensis)dbEST中筛选微卫星位点及引物种间转移扩增[J].海洋与湖沼,2005,36(3):248-254.
- [12]刘必谦,曾庆国,骆其君,等.微卫星标记在坛紫菜丝状体品系DNA指纹构建中的应用[J].水产学报,2005,29(3):323-326.
- [13]Burzycki G M,Waaland J R.On the Position of Meiosisin the Life History of Porphyra torta(Rhodophyta)[J].Botanica Marina,1987,30:5-10.
- [14]Ma J H,Miura A.Observations of the nuclear divisionin the conchospores and their germlings in Porphyrayezoensis Ueda[J].Jap.J.Phycol.,1984,32:373-378.
- [15]Mitman G G.Meiosis,Blade development and sex deter-mination in Porphyra purpurea(Rhodophyta)[J].J.Phycol.,1994,30:147-159.
- [16]Tseng C K,Sun A.Studies on the Alternation of theNuclear Phases and Chromosome Numbers in the LifeHistory of Some Species of Porphyra from China.Botan-ica Marina[J],1989,32:1-8.
- [17]Kitane Y,Whitehouse HL K.Aberrant ascus genotypesfrom crosses involving mutants at the glocus in Sordariafimicola[J].Genet.Res.Comb.,1974,24:229-250.