PCR技术在分子生物学中的应用Application of PCR Technique in Molecular Biology
赵良田,徐迈,杜汉森
摘要(Abstract)：
PCR方法(Polymerase China Reaction),即聚合酶链式反应,又称体外基因倍增,它在分子生物学中有着广泛的应用。文中主要讨论了PCR技术在辅助构建跳跃及连锁文库(Jumping andLinking Library),染色体走步(Chromosome Walking),基因修饰(Rene Modification),重组PCR(Recombinant PCR),足迹法(Footprinting),进行克隆(Cloning)和亚克隆(Subcloning)诸方面的应用,同时指出了PCR技术存在的不足,尚有待于改进。
关键词(KeyWords)： 聚合酶链式反应;分子生物学;跳跃及连锁文库;染色体走步;基因修饰;重组;足迹法;克隆
基金项目(Foundation):
作者(Author): 赵良田,徐迈,杜汉森
DOI: 10.16393/j.cnki.37-1436/z.1997.02.002
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